伏马毒素B1对人脐静脉血管内皮细胞的毒性作用研究

doi:10.11843/j.issn.0366-6964.2017.09.025

摘要/Abstract

摘要：

为探究伏马毒素B₁（FB₁）对人脐静脉血管内皮细胞（HUVEC）的毒性及作用机制，采用不同质量浓度的FB₁处理HUVEC，通过CCK-8法检测细胞活力，流式细胞术检测细胞周期，荧光显微镜观察细胞氧化应激，流式细胞术检测线粒体膜电位变化，Hoechst33342染色检测细胞凋亡，荧光定量PCR检测细胞凋亡相关基因的转录情况。结果显示：FB₁处理组在低质量浓度（2.5、5 μg·mL^-1）短时间（6、12 h）处理后细胞活力变化不显著，超过此剂量和时间细胞活力显著降低（P<0.05或P<0.01），且质量浓度为10、20 μg·mL^-1 FB₁处理组活性氧（ROS）的荧光信号增强；FB₁处理组细胞周期由G₀/G₁期向S期转换时阻滞，细胞线粒体膜电位降低，细胞凋亡相关基因Caspase-3转录升高，Bcl-2转录显著降低（P<0.05），Caspase-9和Bax转录极显著升高（P<0.01）。结果表明，FB₁对人脐静脉血管内皮细胞有毒性作用，能抑制细胞增殖，使细胞周期阻滞在G₀/G₁期；能通过线粒体途径诱导HUVEC凋亡。研究结果为深入研究FB₁对人类细胞的毒性作用机制及相关疾病治疗奠定基础。

Abstract:

To investigate the toxicity and mechanism of action of fumonisin B₁ (FB₁) on human umbilical vein endothelial cells (HUVEC), HUVEC cells were treated with FB₁ in different concentration. The cell vitality, cell cycle, cell oxidative stress, apoptosis, mitochondrial membrane potential change and transcription changes of apoptosis-related genes were detected by Cell Counting Kit-8, flow cytometry, fluorescence microscope, Hoechst33342 staining and real-time quantitative PCR. In result, the vitality of FB₁ treated cells (FB₁ dose exceed 5 μg·mL^-1 and process time exceed 12 h) were significantly decrease (P<0.01 or P<0.05). And the fluorescence intensity of reactive oxygen active (ROS) were enhanced when being treated with 10, 20 μg·mL^-1 of FB₁. FB₁ could prevent HUVEC from G₀/G₁ phase to S phase and reduce the mitochondrial membrane potential. The transcription of apoptosis gene Caspase-3 were increased, Bcl-2 were significantly declined (P<0.05). Caspase-9 and Bax genes transcriptions were significantly increased (P<0.01). The results showed that FB₁ has cytotoxic effect on HUVEC cells, which could inhibit cell proliferation, arrest cell cycle at G₀/G₁ phase and induce apoptosis via mitochondrial pathway. Results in the present works provided the foundations for further research of toxicity and mechanism of FB₁ on human cells and indications for some relevant diseases treatment.

中图分类号:

S859.87

曹喜锋, 秦伟森, 熊永晅, 李楠, 邓衔柏. 伏马毒素B₁对人脐静脉血管内皮细胞的毒性作用研究[J]. 畜牧兽医学报, 2017, 48(9): 1777-1784.

CAO Xi-feng, QIN Wei-sen, XIONG Yong-xuan, LI Nan, DENG Xian-bai. Study on Toxicity of Fumonisin B₁ to Human Umbilical Vein Endothelial Cells[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(9): 1777-1784.

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伏马毒素B₁对人脐静脉血管内皮细胞的毒性作用研究

Study on Toxicity of Fumonisin B₁ to Human Umbilical Vein Endothelial Cells

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